If you require multiple new compensation matrices, simply create a new group and assign it a role of ‘compensation’. There is a single compensation matrix in the workspace by default and it will allow you to create one new compensation matrix in FlowJo. Compensation controls (single stain samples) must now belong to a compensation group in order to create a compensation matrix with these controls. Chapter 2 – Creating a New Compensation Matrix in FlowJo CHAPTER 2 – Creating a FlowJo compensation matrix from single stain controlsīefore compensating data in FlowJo, be sure your parameters which require compensation are scaled correctly.Ĭompensation has been reworked for version 10.
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